Before playing England in their World Cup opener, the Iranian players objected to singing the national anthem.
On the biggest stage in football, fans and players alike staged their anti-government protest.
The Iranian players remained motionless as their national anthem played at Khalifa International Stadium, while the nation's fans erupted in jeers that left some fans in tears.
Chants of ‘Say her name, Mahsa Amini’ rung out throughout the stadium, whilst shirts and banners read ‘Woman, Life, Freedom’.
The middle eastern country has been shaken by months of nationwide protests following the death of 22-year-old woman Mahsa Amini while tin morality police custody.
Amini dies three days after being allegedly arrested for breaching the Islamic republic’s dress code for women, which includes wearing a mandatory headscarf.
The player’s were ultimately praised online for their bravery in taking a stance and standing with the demonstrators against the Iranian regime.
Iran coach Carlos Queiroz asked fans to back off the team, insisting that no matter what the players did they would be crucified.
He told reporters: “Whatever they do, whatever they say, they want to ‘kill’ them.
“Can you imagine whatever you say or do, you’re killed. Let them play the game!”
The Portuguese manager admitted that the political unrest had taken a serious toll on the squad.
He said: “It is not right to come to this World Cup and ask them to do things that are not their responsibility. They want to bring pride and joy for the people.
“You don’t even imagine behind the scenes what these kids have been living in the last few days, just because they want to express themselves as footballers.”
Adding: “Of course, we have feelings and beliefs and in due time, at the right moment, we will express them.”